nm 000261 Search Results


nm 000261  (OriGene)
90
OriGene nm 000261
Nm 000261, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nm 000261/product/OriGene
Average 90 stars, based on 1 article reviews
nm 000261 - by Bioz Stars, 2026-04
90/100 stars
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human myoc  (OriGene)
90
OriGene human myoc
( A ) Western blots for human <t>MYOC</t> protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells <t>were</t> <t>transiently-transfected</t> with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.
Human Myoc, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human myoc/product/OriGene
Average 90 stars, based on 1 article reviews
human myoc - by Bioz Stars, 2026-04
90/100 stars
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myocilin myoc nm 000261 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human myocilin trabecular meshwork inducible glucocorticoid response MYOC Myc DDK tagged
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myocilin myoc nm 000261 human tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles MYOC mGFP tagged Human myocilin trabecular meshwork inducible glucocorticoid response MYOC 200ul 10 7 TU mL
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myocilin myoc nm 000261 human over expression lysate  (OriGene)
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MYOC HEK293T cell transient overexpression lysate as WB positive control
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myocilin myoc nm 000261 human untagged clone  (OriGene)
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MYOC untagged Human myocilin trabecular meshwork inducible glucocorticoid response MYOC
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Image Search Results


( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

Journal: PLoS ONE

Article Title: Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle

doi: 10.1371/journal.pone.0206801

Figure Lengend Snippet: ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

Article Snippet: NTM5 cells at 70 to 80% confluence were transiently-transfected with FLAG-tagged plasmids (8μg total cDNA per 10cm plate), human MYOC (Origene, RC206556; Accession number NM_000261), or mouse Myoc (Origene, MR224777; Accession NM_010865) using FuGENE6 transfection reagent (Promega, E2691).

Techniques: Western Blot, Transgenic Assay, SDS Page, Transfection, Plasmid Preparation